L-RCA (ligation-rolling circle amplification): a general method for genotyping of single nucleotide polymorphisms (SNPs)
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چکیده
منابع مشابه
Rolling Circle Amplification (RCA): an approach for quick detection and identification of fungal species
Conventional methods for fungal identification in the clinical laboratory rely on morphological andphysiological tests. These tests often need several days or weeks to complete and are frequentlyunspecific. Molecular identification mostly implies sequencing, which is relatively expensive andtime-consuming, as well as impractical for large numbers of isolates. The Rolling CircleAmplification app...
متن کاملTyping of multiple single-nucleotide polymorphisms by a microsphere-based rolling circle amplification assay.
The combination of suspension array with rolling circle amplification can lead to a sensitive and specific assay for single-nucleotide polymorphisms (SNPs) detection, as demonstrated in this study. A circular template generated by ligation upon the recognition of a point mutation on DNA targets was amplified isothermally by the Phi29 polymerase on microspheres. The elongation products were labe...
متن کاملrolling circle amplification (rca): an approach for quick detection and identification of fungal species
conventional methods for fungal identification in the clinical laboratory rely on morphological andphysiological tests. these tests often need several days or weeks to complete and are frequentlyunspecific. molecular identification mostly implies sequencing, which is relatively expensive andtime-consuming, as well as impractical for large numbers of isolates. the rolling circleamplification app...
متن کاملMethods for genotyping single nucleotide polymorphisms.
One of the fruits of the Human Genome Project is the discovery of millions of DNA sequence variants in the human genome. The majority of these variants are single nucleotide polymorphisms (SNPs). A dense set of SNP markers opens up the possibility of studying the genetic basis of complex diseases by population approaches. In all study designs, a large number of individuals must be genotyped wit...
متن کاملRolling Circle Amplification (RCA): an approach for quick detection and identification of fungal species
Conventional methods for fungal identification in the clinical laboratory rely on morphological and physiological tests. These tests often need several days or weeks to complete and are frequently unspecific. Molecular identification mostly implies sequencing, which is relatively expensive and time-consuming, as well as impractical for large numbers of isolates. The Rolling Circle Amplification...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2001
ISSN: 1362-4962
DOI: 10.1093/nar/29.22.e116